• Cryopreservation

A procedure used to preserve and store embryos or sperm by deep freezing. The embryos or sperm can be thawed at a later date and used in infertility treatments.

This frozen embryos can be unfrozen, survive and then be transferred to the maternal uterus for implantation and growth, causing a normal pregnancy in all other aspects. See Embryo Cultivation and Transfer.

The main justification of the use and development of Cryopreservation was that not all embryos could be transferred in the same cycle due to the serious risk of multiple pregnancy. In addition, couples with frozen embryos can try additional cycles for a lower cost, increasing their chances of getting pregnant.

Indications:

It is recommended if a greater number of embryos that can be transferred are available.

In the handle of patients with severe OVARIAN HYPERSTIMULATION SYNDROME (OHSS).

Failure in embryo transfer.

• Endometrial preparation for frozen/unfrozen embryo transfer

In this cases, the most important aspect is the endometrial preparation to prepare everthing accordinlgy and syncronically to receive unfrozen embryos after the transfer.

During the Endometrial preparation, progresive dosis of Estradiol are administered and in some cases hormone suppressors like the Luteinizing Hormone, are also administered. To confirm that the endometrium grows adequately, it is necessary to monitor through an ultrasound.

The Luteal Phase supply begins the same amount of days the embryos were frozen; in other words, if the embryos were frozen in the post-fertilization day 3, the administration of Progesterone takes place three days before the embryo transfer.

Cryopreservation of embryos and zygotes has increased the clinical benefits and the accumulated pregnancy rate in a single cycle of Ovarian Stimulation and Embryo Transfer-IVF. Other indications will be the cycles where the ideal endometrial conditions could not be met, either because of premature bled or by an inadequate endometrial development.Until now, all studies that compare pregancies achieved by non frozen embryos against frozen ones, has not found any difference with respect to fetus development, perinatal risks, obstetrics results and congenital abnormalities rate.

• Egg Cryopreservation

Freezing embryos and sperm is a common procedure in the Assisted Reproductive Techniques, but egg freezing has remained in disanvantage due to technial difficulies inherents to the process of this gamete in particular.

The first reports of pregnancies with cryopreserved eggs, appeared more than ten years ago by Chen, Al-Hasani, Porcu and others.

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